Development of a simple and genotype independent in vitro regeneration system in Sugarcane [Saccharum spp] using shoot apex explants

Development of a genotype independent and transformation amenable regeneration system from shoot apex in rice (Oryza sativa spp. indica) using TDZ

Agrobacterium-mediated transformation of indica rice has been established in only a limited number of cultivars because the regeneration of plants from transformed embryogenic calli is highly cultivar-specific. Establishment of a highly efficient plant regeneration system from shoot apex explants applicable to many cultivars of indica rice will accelerate the application of transformation techn...

IN VITRO REGENERATION OF SHOOT AND CORM FROM THE DIF'FERENT EXPLANTS OF CROCUS SATWUS L

Different explants of Crocus sativus L. such as corm, style, ovary, petal, anther and leaf were cultured on LS and MS media supplemented with NAA and BAP or 2,4-D plus BAP at different combinations. Shoot and corm induction occurred in addition to the formation of other organs. Efficient plantlet regeneration with strong roots occurred at 1 mg 1-I of both NAA and BAP. An increase in 2,4-D l...

High-Frequency in Vitro Direct Shoot Regeneration from Nodal Explants of Hyssop Plant (Hyssopus officinalis L.)

Considering great medicinal value of Hyssopus officinalis L. and possibility of its mass production through in vitro culture, two individual experiments was conducted. Effect of various concentrations (0, 2.2, 4.4 and 11 µmol) of TDZ and BAP in combination with 1 µmol of IAA on direct regeneration from nodal explants were assessed. Significant difference between treatments was observed (P≤ 0.01...

The Biology of the Saccharum spp. (Sugarcane)

Development of a system for rapid plant regeneration from invitro sugarcane (Saccharum officinarum L.) meristematic tissue

In this report we describe a methodology for rapid sugarcane shoot regeneration using meristematic tissue from in vitro-grown plantlets. Shoot regeneration from previously induced meristematic tissue, in the presence of up to 22.62 μM of 2,4-D was evaluated. The explants were transferred, after a week induction, to a propagation medium supplemented with activated charcoal and then transferred t...